protein content (Bio-Rad)

Bio-Rad is a verified supplier

Bio-Rad manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Bio-Rad protein content
Protein Content, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 44931 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+standard/Protein+Standard+I/10__22175_slash_mmb__17646-65-1-10
Average 99 stars, based on 44931 article reviews

protein content - by Bioz Stars, 2026-09

99/100 stars

Images

Bradford Assay:

Article Title: Nutritional composition of pollen stores in managed bees across European agro‐ecosystems reveals species‐specific differences but limited pesticide effects
Article Snippet: .. We conducted the Bradford assay with the Bio-Rad Protein Assay Kit microassay 300 μL microplate protocol using bovine γ-globulin as the protein standard (Bio-Rad Laboratories, Inc., Hercules, CA). .. We used three technical replicates for each biological replicate and measured absorbance at 595 nm using a SpectraMax 190 spectrophotometer (Molecular Devices, LLC, Sunnyvale, CA).

Incubation:

Article Title: Comparative analysis of draper mutant alleles and RNAi expression systems in the ovary and brain of Drosophila melanogaster.
Article Snippet: 6 Protein concentration was measured using the Micro BCA Protein Assay Kit (Thermo Fisher 7 Scientific, #23235). .. 50 μg of head protein extracts and 10 μl of protein standard (BioRad, 8 1610374EDU) were mixed with 1X NuPAGETM LDS Sample Buffer (Thermo Fisher Scientific, 9 #NP0007) and incubated at 37 ̊C for 10 minutes. .. 15 μl of each sample were loaded onto a 10 4%-12% NuPAGETM Bis-Tris Mini Protein Gels (Invitrogen, #NP0322BOX).

Nucleic Acid Electrophoresis:

Article Title: Physicochemical, structural, and functional characteristics of protein isolates from New Zealand-grown pea varieties
Article Snippet: SDS loading samples (1 mg/ml PPIs) were prepared in Tricine sample buffer (Bio-Rad) with β-mercaptoethanol (reducing condition) and without β-mercaptoethanol (non-reducing condition). .. These loading samples were heated in a water bath at 100 ◦C for 4 min. For protein separation by gel electrophoresis, 20 μL (20 μg) of the sample and 10 μL of the protein standard (Bio-Rad Precision Plus) were loaded into each well. ..

Protein Concentration:

Article Title: Upregulation of angiotensin-(1–7) formation in human podocytes – enzyme activity assay upon fluid flow shear stress
Article Snippet: Cell suspensions were sonicated for dissolution using Vibra-CellTM (Sonics & Materials Inc., Newtown, CT; US) and centrifugated at 16260 rcf for 10 min at 4°C. .. Supernatants were collected and normalized to total protein concentration determined using BIO-RAD Protein Assay Dye with bovine serum albumin as protein standard (cat. no. 5000006, Hercules, CA, US). ..

Molecular Weight:

Article Title: The Icelandic Mutation in the Murine APP Gene, mAPPA673T, on Amyloid-β Plaque Burden in the 5×FAD Alzheimer Model
Article Snippet: In brief, protein extracts were mixed with 4× Laemmli sample buffer (Bio-Rad Laboratories, Hercules, CA, USA, #1610747) and incubated for 15 minutes at 37 °C. .. Twenty μg protein per lane was loaded onto stainfree 4–15% gradient glycine gels (Bio-Rad Laboratories #4568086) and a protein standard (Bio-Rad Laboratories # 1610376) was loaded onto each gel as molecular weight (MW) marker. .. Proteins were separated in Tris-glycinebuffer (192 mM glycine, 25 mM Tris and 0.9% (w/v) SDS) at 100 V for around 2 hours on ice using a Mini-PROTEAN Electrophoresis Cell (Bio-Rad Laboratories).

Article Title: The Role of the RGD Motif of the IdeC Protein in Streptococcus canis in Adhesion and Invasion.
Article Snippet: .. Precision Plus ProteinTM All Blue Pre-stained Protein Standard was used to determine the molecular weight of bands (BioRad, Hercules, CA, USA, catalogue number 1610373). .. For experiments involving protein-coated beads, SIGMA (Sigma-Aldrich, Saintlouis, MO, USA) fluorescent carboxyl-modified latex beads or SIGMA latex beads were used.

Marker:

SDS Page:

Article Title: Intracellular crowding links dimensionality to cell fate through a mechano-metabolic signalling axis
Article Snippet: A BCA assay (Thermo Scientific) according to manufacturer’s instructions was performed to determine protein concentration. .. SDS-PAGE gels (Bio-Rad) were loaded with 10 μg of protein per condition, and a protein standard (BioRad, Precision Plus Protein TM ) was taken along. .. Following protein separation, the blot was transferred to a membrane using the Trans-Blot transfer pack (BioRad) and the TransBlot Turbo device (BioRad).

Review